Dynamic Plasma Thiol/Disulfide Balance In Experimental Pancreatitis Model In Rats

Abstract

Introduction: Dynamic thiol-disulfide balance has critical roles in regulating antioxidant protection and detoxification.Thiol/disulfite levels, which can be easily measured in blood, were investigated in various proliferative and inflammatory diseases. It was observed that the changes in this balance were correlated with oxidant parameters. In previous studies, it has been shown that oxidative stress increases significantly in acute pancreatitis, and it has been shown that these levels are related to the severity of the disease. Owing to acute pancreatitis is a severe inflammatory condition; the thiol/disulfide balance may correlate with the severity of this disease. Our study investigated thiol/disulfide levels in the experimental pancreatitis model.

Materials and Methods: In our study, 16 Wistar-Albino male rats were used and divided into two groups as experimental and control groups, each group being eight rats. In the experimental group, we generated acute pancreatitis with Cerulein. Amylase, lipase, and thiol/disulfide balance were determined from inferior vena cava blood sampling with median laparotomy. Results: Amylase and lipase levels were found to be significantly different in the experimental group compared with the control group, but there was no significant difference in the plasma thiol/disulfide balance. Conclusion: The study results showed that the plasma thiol/disulfide level did not reveal the severity of acute pancreatitis. In previous studies, thiol/disulfide levels increased in oxidative stress, but this did not happen in our study. The fact that our study was conducted with a small group may have caused this situation. There is a need for more comprehensive studies to investigate in more detail the plasma thiol/disulfide balance.